Dig a small soil pit, at least 10 cm into the mineral soil.
Observe the organic horizons closely.
Determine horizon boundaries and designations and record the horizons on your tally sheet. Possible horizons are L, F, H and Ah.
Decide if you are going to split the horizons to provide more detail. For example, you might have a Ln and a Lv horizon. The Ln is new litter and the Lv is decayed litter.
Pay particular attention to determining the type of F horizon as this will be used to classify your humus form. You may designate Fm, Fz or Fa.
Record the horizon depths using the mineral/organic interface as your 0 point. For example, L (7-5 cm), F (5-2 cm), H (2-0 cm), Ah (0-2 cm).
You can then use the methods of humus form assessment to record other properties such as moisture status, color, fabric, consistence, character, and what biota you see in the sample. You can also indicate if you find con-conforming materials such as decaying wood, coarse fragments or charcoal.
You may carry out your description in the field or after recording horizon designations and depths, you can collect an intact sample to take to the lab for further assessment.
The depth of organic horizons is measured upward from zero depth, with zero being the interface between the organic and mineral horizons. In organic soils, the depth is measured downward from the top of the profile, and this is taken as zero depth.
Boundary Distinctness
Class
Description
Abrupt
< 5mm
Clear
5 – 10mm
Gradual
11 – 20mm
Diffuse
> 20mm
Boundary Form
Class
Description
Smooth
Nearly a plane
Wavy
Pockets wider than deep
Irregular
Pockets deeper than wide
Broken
Discontinuous, some parts are unconnected
Moisture Status
Some morphological properties of materials described may be affected by their moisture content.
Class
Description
Desiccated
Extremely dry condition; organic tissues will crack or snap when broken or crushed
Dry
Moisture is not apparent; material will not rub colour out on fingers
Moist
Moisture is apparent; colour will rub out on fingers; if material is squeezed in hand, no water will be observed (generally below field capacity)
Wet
If material is squeezed or rubbed in hand, water will be observed (generally below field capacity)
Saturated
Water is observed without squeezing or rubbing of material
Color
The colour of materials within the humus form profile may be of less interpretive significance than that for mineral soil horizons. However, the Munsell Color Chart can also be used to assess the colour of organic horizons.
Fabric
The term fabric has been applied to the description of structure, consistence and character of organic materials in the humus profile.
Structure
Classified according to grade, type, kind and size of the macromorphological aggregation of the material within a horizon or layer.
Grade of Structure
Class
Description
Weak
Disaggregated materials are dominant; <20% distinction of aggregation
Moderate
Some disaggregated material is found; 20 – 60% distinction of aggregation
Strong
Aggregated materials are dominant; most material conforms to the same arrangement; >60% distinction of aggregation
Consistence
A measure of the strength and nature of forces combining materials together, assessed by deformation or rupture when pressure is applied.
Class
Description
Loose
There is no consistence of the material
Friable
A material that crumbles easily under gentle pressure
Firm
A material that can be crushed under moderate pressure; resistance is noticeable
Pliable
Material is soft and plastic
Resilient
Material that is springy or elastic and assumes original state after forces of deformation have been applied and released
Tenacious
Material is cohesive, not easily pulled apart
Character
The distinctive quality, or tactility: how the material feels.
Class
Description
Mushy
Materials are wet or saturated, soft and spongy
Mucky
Materials are usually wet, smooth and sticky; they contain silt and clay sized mineral particles
Greasy
Materials are smooth and greasy when moist and easily workable; fine mineral particles are usually absent
Gritty
Refers to a rough tactility produced by mineral granules or coarse fragments
Leafy
Refers to the tactility of materials produced by deciduous foliage showing a shingle-like layering (banded structure)
Mossy
Refers to the tactility produced by bryophytes with more or less preserved vegetative structures
Acerose
Refers to the tactility produced by bryophytes with more or less preserved vegetative structures
Felty
Refers to the tactility produced by abundant fungal mycelia
Fibrous
Refers to tactility produced by and abundance of fibrous plant residues that do not break down upon rubbing (roots)
Ligneous
Refers to the tactility produced by coniferous or deciduous wood fibres
Crusty
Refers to a hard and brittle tactility of dry or desiccated materials
Roots
For fine and very fine roots, the surface area of the profile viewed is 2.5cm X 2.5cm while for mediuem, coarse and v. coarse roots, a 25cm X 25cm area is viewed. Both living and dead roots are counted, unless it is very clear that a root is dead.
Root Abundance
Class
Number of roots/unit surface area
Very few
< 3
Few
3 – 10
Common
11 – 20
Plentiful
21 – 30
Abundant
> 30
Root Size
Class
Size (diameter in mm)
Very fine
< 1
Fine
3 – 10
Medium
11 – 20
Coarse
21 – 30
Very coarse
> 30
Root Orientation
Class
Description
Random
Roots are oriented in all directions
Oblique
Roots are oriented along oblique planes
Horizontal
Roots are oriented along horizontal planes
Vertical
Roots are oriented along vertical planes
Non-Conforming Materials
Usually charcoal, decaying wood and mineral coarse fragments. The kind, distribution, size and abundance are described. They may have: Regular Distribution throughout, or Irregular Distribution only in certain parts of the horizon. Abundance refers to the volume by percentage occupied by non-conforming materials for each size class.
Distribution
Class
Description
Random
Materials are distributed randomly; there is no recognizable, specific pattern of distribution
Clustered
Materials are distributed in clusters or groups
Banded
Materials are distributed in bands, sheets or layers
Size
Class
Length (mm)
Very fine
< 5
Fine
5 – 20
Medium
21 – 100
Coarse
101 – 500
Very coarse
> 500
Abundance
Class
Description
Few
Occasional and scatted pieces; volume < 5%
Common
Frequent occurrence; volume 5 – 20%
Many
Numerous, coarse pieces; volume > 20%
Biota
The activity of soil flora and fauna paly such an important role in humus formation that their description cannot be overlooked.
Soil Fauna
Soil fauna are those that pass one or more active stages wholly or largely in the soil or surface litter, excluding those species which occur there only in passive stages such as eggs, cysts or pupae.
Direct observation of fauna or indirect observation through the presence of casts can be used to determine faunal presence – castes provide a good indication of their activity
Size Distribution, shape, colour and level of humification indicate the type of soil fauna that produced casts.
Mite-type: Small (<0.1mm diameter) spherical/oval, humified, rust to dark brown, lacking mineral grains
Enchytraeid-type: Small (0.05-0.2mm diameter) sub-spherical, rugose, generally well-humified, brown with varying amounts of mineral grains and clay
Arthropod-type (and small surface-feeding earthworms): Visible without hand lens (1-3mm long) well-humified, dark brown, containing mineral grains but low in clay
Worm casts: Well-humified, generally brown or greyish brown, containing mineral grains and clay, spongy or coarse rugose granular structure (5-10mm diameter)
A. Mites (Acarina) B. Springtails (Collembola) C. Spiders (Areneida) D. Fly larvae (Diptera) E. Beetles and Larvae (Coleoptera) F. Ants (Hymenoptera) G. Woodlice (Isopoda) H. Centipedes and millipedes (Myriapoda) I. Termites (Isoptera) J. Earthworms (Lumbricida) K. Potworms (Enchytraeida) L. Nematodes (Nematoda)
Soil Fauna – Sizes
Class
Description
Macrofauna
Animals with a body size greater than 1cm (in longest dimension); earthworms, vertebrates, molluscs and large arthropods
Mesofauna
Animals ranging in body size from 1cm to 0.2mm including some mites, springtails, potworms and most of the larger nematodes. The lower limit is about the limit of viewing with a 10x hand lens.
Microfauna
Organisms less than 0.2mm in size; includes the protozoa as well as many of the smaller mites and nematodes
Droppings – Abundance
Class
Description
None
No visible droppings
Few
Droppings occasional observed but scattered
Common
Droppings commonly observed
Abundant
Droppings frequently observed in relatively large numbers throughout the horizon
Droppings – Distinction
Class
Description
Random
Droppings are distributed randomly, there is no recognizable pattern of distribution
Clustered
Droppings are distributed in clusters or groups
Banded
Droppings are distributed in bands, sheets or layers
Soil Flora
Bacteria, actinomycetes, algea and fungi. Description is usually narrowed to fungi in the field. Fungi include: Rusts, moulds, yeasts and mushrooms. Presence is detected by observing a mass of hyphae (thread-like filaments) called mycelium. Colours include brown, black, grey, white, red, yellow and blends of these or transparent. Description includes abundance, colour and distribution.
Fungal Mycelia – Abundance
Class
Description
None
Mycelia not visible
Few
Mycelia occasionally present but scattered and not easily observed
Common
Mycelia commonly observed
Abundant
Mycelia observed continuously throughout the horizon, often “matting” materials together and creating a “felty” tactility
Fungal Mycelia – Distribution
Class
Description
Random
Mycelia are distributed randomly, there is no recognizable pattern of distribution
Clustered
Mycelia are distributed in clusters or groups
Banded
Mycelia are distributed in bands, sheets or layers